Samples of Pa DDAH, at concentrations of 0.5, 2.0, 6.0 and 12 mg ml −1 in 25 mM Tris, 100 mM NaCl, pH 7.0, 5 mM DTT buffer, were passed through 0.2 μm membrane filters (Whatman) during injection.

Digital Terrestrial Television (DTT) in Portugal has been a “costly failure”, currently being used by less than 3 per cent of the country’s population and costing RTP €8 million annually. Speaking ...

Buffer optimization may be used to improve sample stability ... If you have free thiols reducing agents, DTT or TCEP (1-5mM), may also be needed.

Transcription activation of genes by estrogens is driven by enhancers, which are often located within the same Topologically Associating Domain (TAD) as non-targeted promoters. We investigated how ...