Samples of Pa DDAH, at concentrations of 0.5, 2.0, 6.0 and 12 mg ml −1 in 25 mM Tris, 100 mM NaCl, pH 7.0, 5 mM DTT buffer, were passed through 0.2 μm membrane filters (Whatman) during injection.

The so-called serviceability buffer – which forces banks to evaluate the borrowing capacity of a customer by adding 3 per cent to the advertised interest rate – should be cut for first home ...

Buffer optimization may be used to improve sample stability ... If you have free thiols reducing agents, DTT or TCEP (1-5mM), may also be needed.

This valuable study reveals extensive binding of eukaryotic translation initiation factor 3 (eIF3) to the 3' untranslated regions (UTRs) of efficiently translated mRNAs in human pluripotent stem ...

Transcription activation of genes by estrogens is driven by enhancers, which are often located within the same Topologically Associating Domain (TAD) as non-targeted promoters. We investigated how ...